Touch imprint cytology of leaves (Vigna radiata) with and without fine iron particles and Prussian Blue staining

Benjamin J. Scherlag, Bing Huang, Ling Zhang, Kaustuv Sahoo, Abraham A. Embi, Sunny S. Po

Heart Rhythm Institute University of Oklahoma Health Sciences Center Oklahoma City, Oklahoma, USA


Background: Touch Imprint Cytology, in which tissues are applied to glass slides and stained, has become a useful method for the examination of various types of tumors that need to be studied and diagnosed peri-operatively. Methods: In the present report, we used touch imprinting applied to leaves from the common Mung bean plant (Vigna radiata).Isolated leaves (n=100) were immersed in nano-sized iron particle solutions for 1 hour and the placed between 2 glass slide containing a small amount of aliquots of the iron particle solution plus a specific iron stain Prussian Blue. For comparison, leaves were immersed in deionized water and “sandwiched” between glass slides containing the same medium (n=10). After 12- 24 hour the tissues were peeled off the glass surfaces and examined by optical microscopy. Results: The experimental set, consistently showed outlines of cell types specific for the epidermal and underside of leaves. Specifically, guard cell surrounding stoma were prevalent on the underside of the leaf, whereas trichomes and cell walls of epidermal cells were noted on the upper surface. The control set showed only imprints of the leaf edges. Conclusions: Using iron particles and iron staining solutions, touch imprints of several cell types found on the two leaf surfaces of the Mung bean plant were imaged. Evidence was presented that the lignin in plant cell walls have a strong affinity for iron and that inherent electromagnetic energy derived from metabolism may also contribute to the attraction of  iron particles to imaging leaf cytology. Journal of Nature and Science, 1(1):e32, 2015.

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