Genetic diversity and paternal analysis of open-pollinated progenies of Larix olgensis seed orchard

Zhigang Wei, Zianshang Qu, Cong Hou, Yingying Liu, Lijie Zhang, Chuanping Yang, Hairong Wei

Michigan Technological University


280 open pollinated progenies (seeds) and their parents, 49 paternal trees and 7 maternal trees that were planted in an open-pollinated seed orchard of Larix olgensis (Henry) at Qingshan Forest Farm, Heilongjiang Province,P. R. China, were studied by simple sequence repeats (SSR) markers. The genetic diversity between the parents and progenies, paternal pollen contribution to the progenies, the effect of crown-facing sides to pollination and pollen disperse distance were investigated. We found that the genetic diversity difference between the progenies and parents were not significant. For example, the average fixed indices of parental and progeny population were 0.0071 and 0.0063 respectively.  In addition, no obvious fixed mating patterns were found in the seed orchard though the pollen contribution of each paternal parent was different, implying that some maternal parents could only be pollinated by few paternal trees. Interestingly, two alleles present only in five progenies that accounted for 3.09% of all progenies were not from any 49 paternal parents, suggesting there was invasion of foreign pollen in seed orchard. We also found the main effective pollen dispersal distance of L. olgensis varied from15 to 95 m. However, we did not observe significant correlation between effective pollen dispersal distance and male reproductive fitness in the seed orchard. Moreover, we found that the crown-facing sides of maternal trees had some influence on the paternal constitution of progenies, which, however, was not at significant level. Our study provided valuable information for more effective management of seed orchard and viable long-time breeding strategies to ameliorate L. olgensis or other coniferous species. Journal of Nature and Science, 1(1):e19, 2015.

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