Investigation of Genipin-crosslinked Hydroxybutyl Chitosan Polymeric Stent for Cardiovascular Diseases

Jiao-Jiao Bing, Chao Feng, Yao-Yao Yan, Guo-Hui Sun, Xiao-Jie Cheng, Ming Kong, Ya Liu, Chang-Qing Jiang, Jing-Jing Li, Xi-Guang Chen

College of Marine Life Science, Ocean University of China, Qingdao 266003, China. College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China

A limitation of the use of polymers as stent matrices is their inherent weak mechanical strength. To reinforce the mechanical strength of the hydroxybutyl chitosan (HBC) stent, a genipin- crosslinked hydroxybutyl chitosan polymeric stent was developed. The inner structure of the HBC-genipin stent (HBC-G) was long narrow strip structure, superior to the porous structure of its counterpart, the chitosan-genipin stent (CS-G). With the increase of the crosslinking time, the crosslinking degree increased whereas the self-expansion ratio decreased. The amount of BSA absorption in the HBC-G was 48.9 ± 4.2 μg/mL, about 62.3% of that in the CS-G (78.5 ± 5.6 μg/mL). The hemolysis rate of HBC-G was under 5% and lower than the CS-G, and, which was in line with the national standard. The viability of the human umbilical vein endothelial cells (HUVECs) was above 80% at any concentration of the HBC-G, demonstrating that the stent possesses good blood compatibility and cytocompatibility to HUVECs. The results of degradation test in vitro indicated that the degradation rate of stent was associated with the crosslinking degree. HBC stent with 3 hours crosslinking time could be more easily degraded. These results provided evidence that HBC-G is exciting and promising for an alternative cardiovascular stent. Journal of Nature and Science, 1(5):e100, 2015

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