Journal of Nature and Science (JNSCI), Vol.3, No.12, e476, 2017

Genetics

 

Histone acetyltransferase KAT8 and oocyte development

 

Shi Yin1,2,#, Xiaohua Jiang2,#, Tej K. Pandita3, and Qinghua Shi2,*

 

1College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China. 2Molecular and Cell Genetics Laboratory, The CAS Key Laboratory of Innate Immunity and Chronic Diseases, Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, CAS Center for Excellence in Molecular Cell Science, University of Science and Technology of China, Collaborative Innovation Center of Genetics and Development, Collaborative Innovation Center for Cancer Medicine, Hefei ,China. 3Department of Radiation Oncology, The Houston Methodist Research Institute, Houston, TX 77030, USA


Oocyte development is necessary for female fertility and is characterized by dramatic changes in gene expression and chromatin structure. Histone acetylation is an important epigenetic mechanism for gene regulation as it changes the chromatin structure and affects the binding ability of  transcription factors to DNAs. Histone acetylation level is regulated antagonistically by two classes of enzyme, histone acetyltransferases (HATs) and histone deacetylases (HDACs). In this review, we briefly summarize the current knowledge about an important HAT, K (Lysine) Acetyltransferase 8 (KAT8), and introduce the role of this enzyme in oocyte and follicle development, which gives new insight about epigenetic and female reproduction.

 

Kat8 | histone acetylation | oocyte | follicle | reproduction

 

Histone acetylation and oocyte development

Oogenesis plays an irreplaceable role in female fertility as it determines the production of healthy eggs (1). To ensure the normal development of oocytes, accurate gene expression and appropriate chromosome configuration are needed (2, 3). Among the various post-translational modifications, histone acetylation is one of the most well-studied modifications. Acetylation regulates the chromatin conformation between open and closed state by changing the electrostatic affinity between histones and DNA, thus affecting the binding ability of transcription factors to DNAs (4). Histone acetylation level is regulated by two classes of enzymes, histone acetyltransferases (HATs) and histone deacetylases (HDACs) (5-7). HATs are responsible for adding acetyl groups to lysines to neutralize the positive charge on histones. As a result, the electrostatic affinity between histone and DNA is weakened and chromatin maintains an open structure for factors that promote transcription (4). In contrast, HDACs are mainly required for the removal of acetyl groups. This removal causes excess positive charges on histone, which contributes to strong electrostatic affinity between histones and negatively charged DNAs, then compacts the chromatin structure and represses gene expression (8).

Several HDACs have been reported necessary for oocyte and follicle development. For example, knockdown Sirt6 in germinal vesicle (GV) stage mouse oocytes by RNA interference results in abnormal spindle morphology and chromosome alignment, as well as elevated incidence of aneuploidy. Consequently, oocyte maturation is disrupted with fewer first polar bodies being released (9). Conditional knockout of another HDAC-encoding gene, Hdac2, in mouse oocyte causes similar oocyte maturation defects as Sirt6 knockdown in oocytes. In addition, the knockout mice contain fewer antral follicles compared with that of control (10). Disrupted follicle development is also observed in oocytes of Hdac1 and Hdac2 double conditional knockout mice. The mutant mice are interfile with small ovarian size and disrupted oogenesis (5). It is worth mentioning that oocytes lack Sirt6 or Hdac2 display specific increase in H4 Lys 16 acetylation (H4K16ac) levels (9, 10), suggesting a potential role of H4K16ac in oogenesis.

Members of the HATs, family comprised of the MYST, Gcn5/PCAF and p300/CBP subfamilies function as gene co-factors (4). However, compared with the well-studied roles of HDACs in oogenesis, how HATs regulate oogenesis is poorly understood. Here, based on our recent report, we briefly introduce a HAT, K (Lysine) Acetyltransferase 8 (KAT8, also known as MOF or MYST1) (11-13), and summarize the experiments results of this conditional gene knockout mice to elaborate the roles of KAT8 in oocytes and follicle development.

 

Histone acetyltransferase KAT8

K (Lysine) Acetyltransferase 8 (KAT8, also known as MOF or MYST1) is a a HAT protein that is conserved among multiple species and expressed widely in different tissues (11-13). It is a histone acetyltransferase belonging to the MYST family (Moz-Ybf2/Sas3-Sas2-Tip60) (14) that contains a conserved acetyl-CoA-binding motif, a zinc finger domain (15-17) and a chromo domain (18). This acetyltransferase acetylates histone substrates via two complexes, male-specific lethal (MSL) and non-specific-lethal (NSL) (19-21). Though the NSL complex can acetylate histone H4 at lysines 5 and 8 (22), the major histone substrate for KAT8 is H4K16 (23, 24). KAT8 also acetylates non-histone substrate, such as p53 at lysine 150. Acetylated p53 promotes the expression of several pro-apoptotic factors, such as PUMA and BAX (25). In addition, KAT8 can regulate its own activity by acetylating itself at  lysine 274 (26).

KAT8 is involved in multiple biological process, such as transcription, DNA damage repair, apoptosis and metabolism. In Drosophila, MOF (KAT8) is a key component of the X chromosome dosage compensation complex (27), which is essential for balancing the expression of X-linked gene between male and female (14, 28, 29). Microarray analysis showed that several key genes, such as human leukocyte antigen complex P5 (HCP5), are significantly down-regulated in KAT8 knockdown HeLa cells (30). Similarly, in Kat8 knockout mouse embryonic stem cells, levels of several critical transcription factors, such as Oct4, Nanog and Sox2 are significantly decreased and these genes are directly targeted by KAT8 (31). Once DNA damage occurs in cells, ataxia-telangiectasia-mutated (ATM) can phosphorylate KAT8 at the T392 site, then the phosphorylated KAT8 could colocalize with -H2AX, ATM and p53BP1 foci to recruit BRCA1 and MDC1 (32, 33). The role of Kat8 in apoptosis is contradictory. For example, in H1299 cells, KAT8 can acetylate p53 to activate the apoptotic pathway (25), while Kat8 may play an anti-apoptotic role as in Kat8 knockout mouse embryos, many cells show features of apoptosis including Caspase3 activation and DNA fragmentation (34). These results suggest that Kat8 may play diverse roles in apoptosis via different pathways. Kat8 is also involved in metabolism by binding to mitochondrial DNAs and regulating their expression. Serious mitochondrial degeneration, high-energy consumption, as well as defective oxidative phosphorylation could be observed after Kat8 deletion in mouse cardiomyocytes, which indicates that Kat8 plays an important role in connecting epigenetics with metabolism (35).

 

 


Figure 1. Working model of how oocyte and follicle development is regulated by KAT8. KAT8 and H4K16ac bind to promoters of several antioxidant genes and promote their expression. ROS levels are subsequently decreased, which leads to the prevention of oocyte apoptosis and follicle atresia.

 

 


Expression of KAT8 during mouse oocyte development

During mouse folliculogenesis, Kat8 expression in oocytes exhibits temporal and spatial patterns. Real-time PCR showed that the level of Kat8 transcript increases slightly from 5 days postpartum (5d) to 14d, subsequently reaches the highest level at full-grown GV stage, then decreases rapidly during metaphase I and metaphase II. Immunocytochemical analysis proved that the protein localizes in nuclei from 5d to full-grown GV stage and is uniformly dispersed throughout the entire upon germinal vesicle breakdown (GVBD) (36). Interestingly, H4K16ac, the major histone substrate for KAT8, also displays a similar expression profile to KAT8 as the nuclear staining of H4K16ac increased from 5d to GV stage and decreased significantly upon GVBD (36).

 

KAT8 is essential for mouse oocyte and follicle survival

Based on the fact that global ablation of KAT8 causes embryonic lethality (37), specific CRE recombinase (Gdf9-Cre) mediated deletion was performed to reveal the function of this acetyltransferase in mouse oocyte. Mice lacking KAT8 in oocytes are infertile with smaller ovarian size. Moreover, the Kat8 conditional knockout (cKO) mice contain fewer full-grown GV stage oocytes with small sizes and defective morphologies. Follicle development is also disrupted as much fewer secondary, preantral and antral follicles are observed in the cKO mice compared to control (36). The proliferation of granulosa cells is not affected based on BrdU incorporation and PCNA immunostaining, however, higher incidences of apoptosis and DNA damage in oocytes of  secondary or later stage follicles are observed after Kat8 deletion (36). All these results indicate that KAT8 is necessary for oocyte and follicle development.

 

KAT8 regulates ROS levels by mediating antioxidant genes expression in oocytes

Oocyte development is characterized by dramatic changes in gene expression (38). Whole transcriptome sequencing analysis elaborates that expressions of 858 transcripts are altered after Kat8 deletion in oocytes, including 500 up-regulated and 358 down-regulated genes (36). Interestingly, several antioxidant genes, including peroxiredoxin 1 (Prdx1), Prdx2, glutathione peroxidase 1 (Gpx1), Gpx4 and Gpx6 (39, 40) are  significantly down-regulated in Kat8-defecient oocytes. Consistent with this, increased reactive oxygen species (ROS) levels are observed in Kat8 knockout oocytes. Chromatin immunoprecipitation (ChIP) analysis shows that some of these genes are directly regulated by KAT8 (36), which indicates the down-regulation of antioxidant genes is directly caused by Kat8 deletion. Generally, to ensure the normal oocyte development, excessive ROS levels should be removed by endogenous antioxidant enzymes (41). Thus, we hypothesize that when Kat8 is deleted in the oocytes, accompanied with decreased expression of antioxidant genes, the evaluated ROS levels could disrupt oocyte development. Indeed, ROS levels are down-regulated  significantly in Kat8-defecient oocyte after intraperitoneal injection of N-acetylcysteine (NAC), a widely used antioxidant (42-44) to the cKO mice. The ovarian size is larger in cKO mice injected with NAC compared to those injected with PBS. Oogenesis is also improved in NAC-treated group. Moreover, more secondary, preantral and antral follicles are observed in NAC-treated cKO mice. Consistently, both the incidences of apoptosis and DNA damage in Kat8 knockout oocyte are decreased after NAC administration (36). Thus, histone acetyltransferase KAT8 is essential for mouse oocyte development by regulating ROS levels.

 

Conclusion

Here we summary the recent studies of histone acetylation in relation to oogenesis and focus on an important acetyltransferase KAT8. This conserved enzyme functions widely in multiple biological process and is highly expressed in full-grown GV stage oocytes. KAT8 in oocytes is specially responsible for H4K16ac and it can directly bind to the promoter of several key antioxidant genes to mediate their expression. The cellular ROS levels are consequently decreased, thus maintaining the environment favorable for the normal oocyte and follicle development (Fig.1). Of note, as a gene co-factor, various pathways are affected upon Kat8 deletion, whether and how these pathways affect oocyte development need to be further confirmed. In conclusion, the study of Kat8 on oocyte development supplies new information about how epigenetic factors regulate female reproduction and may provide new solutions to human oogenesis or folliculogenesis problems.

 

Acknowledgments

The authors wish to acknowledge support from the National Key Research and Developmental Program of China (2016YFC1000600), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB19000000), the National Natural Science Foundation of China (31501199, 31671557, 31501203 and 31371519), Innovation Team Project for Conservation and Utilization of Yak Genetic Resources (13CXTD01), NIH RO1 CA129537 and RO1 GM109768.

 

 


 


1.  Rodrigues P, Limback D, McGinnis LK, Plancha CE, & Albertini DF (2008) Oogenesis: Prospects and challenges for the future. J Cell Physiol 216(2):355-365.

2.  Jagarlamudi K & Rajkovic A (2012) Oogenesis: transcriptional regulators and mouse models. Mol Cell Endocrinol 356(1-2):31-39.

3.  van den Hurk R & Zhao J (2005) Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. Theriogenology 63(6):1717-1751.

4.  Gu L, Wang Q, & Sun QY (2010) Histone modifications during mammalian oocyte maturation: dynamics, regulation and functions. Cell Cycle 9(10):1942-1950.

5.  Ma P, Pan H, Montgomery RL, Olson EN, & Schultz RM (2012) Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A 109(8):E481-489.

6.  Kouzarides T (2007) Chromatin modifications and their function. Cell 128(4):693-705.

7.  Haberland M, Montgomery RL, & Olson EN (2009) The many roles of histone deacetylases in development and physiology: implications for disease and therapy. Nature reviews. Genetics 10(1):32-42.

8.  Shahbazian MD & Grunstein M (2007) Functions of site-specific histone acetylation and deacetylation. Annu Rev Biochem 76:75-100.

9.  Han L, et al. (2015) Sirt6 depletion causes spindle defects and chromosome misalignment during meiosis of mouse oocyte. Sci Rep 5:15366.

10.  Ma P & Schultz RM (2013) Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse. PLoS Genet 9(3):e1003377.

11.  Gupta A, et al. (2013) T-cell-specific deletion of Mof blocks their differentiation and results in genomic instability in mice. Mutagenesis 28(3):263-270.

12.  Thomas T, Loveland KL, & Voss AK (2007) The genes coding for the MYST family histone acetyltransferases, Tip60 and Mof, are expressed at high levels during sperm development. Gene Expr Patterns 7(6):657-665.

13.  Rea S, Xouri G, & Akhtar A (2007) Males absent on the first (MOF): from flies to humans. Oncogene 26(37):5385-5394.

14.  Hilfiker A, Hilfiker-Kleiner D, Pannuti A, & Lucchesi JC (1997) mof, a putative acetyl transferase gene related to the Tip60 and MOZ human genes and to the SAS genes of yeast, is required for dosage compensation in Drosophila. The EMBO journal 16(8):2054-2060.

15.  Yang Y, Han X, Guan J, & Li X (2014) Regulation and function of histone acetyltransferase MOF. Frontiers of medicine 8(1):79-83.

16.  Avvakumov N & Cote J (2007) The MYST family of histone acetyltransferases and their intimate links to cancer. Oncogene 26(37):5395-5407.

17.  Pillus L (2008) MYSTs mark chromatin for chromosomal functions. Current opinion in cell biology 20(3):326-333.

18.  Su J, Wang F, Cai Y, & Jin J (2016) The Functional Analysis of Histone Acetyltransferase MOF in Tumorigenesis. Int J Mol Sci 17(1).

19.  Mendjan S, et al. (2006) Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila. Mol Cell 21(6):811-823.

20.  Smith ER, et al. (2005) A human protein complex homologous to the Drosophila MSL complex is responsible for the majority of histone H4 acetylation at lysine 16. Mol Cell Biol 25(21):9175-9188.

21.  Taipale M, et al. (2005) hMOF histone acetyltransferase is required for histone H4 lysine 16 acetylation in mammalian cells. Mol Cell Biol 25(15):6798-6810.

22.  Cai Y, et al. (2010) Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. J Biol Chem 285(7):4268-4272.

23.  Morales V, et al. (2004) Functional integration of the histone acetyltransferase MOF into the dosage compensation complex. The EMBO journal 23(11):2258-2268.

24.  Li X, Wu L, Corsa CA, Kunkel S, & Dou Y (2009) Two mammalian MOF complexes regulate transcription activation by distinct mechanisms. Mol Cell 36(2):290-301.

25.  Sykes SM, et al. (2006) Acetylation of the p53 DNA-binding domain regulates apoptosis induction. Mol Cell 24(6):841-851.

26.  Sun B, et al. (2011) Regulation of the histone acetyltransferase activity of hMOF via autoacetylation of Lys274. Cell research 21(8):1262-1266.

27.  Belote JM & Lucchesi JC (1980) Male-specific lethal mutations of Drosophila melanogaster. Genetics 96(1):165-186.

28.  Deng X, et al. (2013) Mammalian X upregulation is associated with enhanced transcription initiation, RNA half-life, and MOF-mediated H4K16 acetylation. Developmental cell 25(1):55-68.

29.  Akhtar A & Becker PB (2000) Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila. Mol Cell 5(2):367-375.

30.  Liu N, et al. (2013) A potential diagnostic marker for ovarian cancer: Involvement of the histone acetyltransferase, human males absent on the first. Oncology letters 6(2):393-400.

31.  Li X, et al. (2012) The histone acetyltransferase MOF is a key regulator of the embryonic stem cell core transcriptional network. Cell Stem Cell 11(2):163-178.

32.  Li X, et al. (2010) MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1. Mol Cell Biol 30(22):5335-5347.

33.  Gupta A, et al. (2014) MOF phosphorylation by ATM regulates 53BP1-mediated double-strand break repair pathway choice. Cell Rep 8(1):177-189.

34.  Thomas T, Dixon MP, Kueh AJ, & Voss AK (2008) Mof (MYST1 or KAT8) is essential for progression of embryonic development past the blastocyst stage and required for normal chromatin architecture. Mol Cell Biol 28(16):5093-5105.

35.  Chatterjee A, et al. (2016) MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria. Cell 167(3):722-738 e723.

36.  Yin S, et al. (2017) Histone acetyltransferase KAT8 is essential for mouse oocyte development by regulating ROS levels. Development 144(12):2165-2174.

37.  Gupta A, et al. (2008) The mammalian ortholog of Drosophila MOF that acetylates histone H4 lysine 16 is essential for embryogenesis and oncogenesis. Mol Cell Biol 28(1):397-409.

38.  Pan Z, et al. (2012) Current advances in epigenetic modification and alteration during mammalian ovarian folliculogenesis. J Genet Genomics 39(3):111-123.

39.  Brigelius-Flohe R & Maiorino M (2013) Glutathione peroxidases. Biochim Biophys Acta 1830(5):3289-3303.

40.  Rhee SG (2016) Overview on Peroxiredoxin. Mol Cells 39(1):1-5.

41.  Tiwari M, et al. (2015) Apoptosis in mammalian oocytes: a review. Apoptosis 20(8):1019-1025.

42.  Danilovic A, et al. (2011) Protective effect of N-acetylcysteine on early outcomes of deceased renal transplantation. Transplant Proc 43(5):1443-1449.

43.  Inci I, et al. (2007) N-acetylcysteine attenuates lung ischemia-reperfusion injury after lung transplantation. Ann Thorac Surg 84(1):240-246; discussion 246.

44.  Usta U, et al. (2008) Tissue damage in rat ovaries subjected to torsion and detorsion: effects of L-carnitine and N-acetyl cysteine. Pediatr Surg Int 24(5):567-573.


 



Conflict of Interest: No conflicts declared.

* Corresponding Author. Email: qshi@ustc.edu.cn

#These authors contributed equally to this work.

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