A Single Cell Assay for Quantifying Extracellular Vesicles from Single Cells

Yu-Jui Chiu, Wei Cai, Yihuan Tsai, Yu-Hwa Lo


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Heterogeneity of a cell population has been considered a fundamental property of cellular system. Single cell analysis is essential to understand the variation within a heterogenous cell population; however, most existing single cell analysis methods can only provide a glimpse of cell property at specific time point, unable to provide phenotypic information varying in time. Here we review single cell analysis assays we developed to monitor single cell behaviors and cell secretions over time. The assays also provide the capability of translocating cells to another substrate for downstream analysis of selected single cells.

J Nat Sci, 5(1):e547, 2019




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