Electromagnetic Imaging of Subdermal Human Hair Follicles In Vivo

Benjamin J Scherlag, Kautsuv Sahoo

Heart Rhythm Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma, USA Corresponding Author: Benjamin J. Scherlag, PhD. 1200 Everett Drive (6E 103), Oklahoma City, OK 73104, USA. E-mail: benjamin-scherlag@ouhsc.edu

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Background: Previously we used a method for imaging electromagnetic energy emitted human hairs removed from the skin. In the present report the same methodology was able to record images of sub-dermal hair follicles.

Methods: A relatively flat area of the author’s forearms was covered by a strip of aluminum foil and taped to the area. A glass coverslip, placed so that a portion of the coverslip lay over the aluminum strip and a part positioned over the uncovered skin. A drop of a solution of iron nano-particles was put on the glass coverslip and a second coverslip placed on top to create a uniform liquid sandwich.  A small strip of tape at each end of the coverslip was attached to the forearm so that the entire preparation could be maintained without movement for 4-6 hours (n=10). For comparison, hairs with attached follicles were plucked from the skin and placed in a glass slide sandwich (n=9). Images from both groups were viewed with an optical microscope and microphotographs made with a camera attachment. Quantitative comparisons of the follicle areas in each group was determined using the measurement tools of the Pro-plus software program.

Results: Sub-dermal images showed characteristic follicle structures and were, for the most part, similar in size and shape to the ex vivo images. Quantitative comparisons showed a significant difference (p=0.02, Table 1) between area measurements of in vivo and ex vivo imaged follicles. By adjusting for differences by which images were obtained, no significant area differences were then found (p=0.9, Table 2).

Conclusions: Using a simplified method consisting of a solution containing nano-sized iron particles and an iron stain, we were able to record images of follicles under the shaved and unshaved skin of the forearm. Comparison of these images and those of follicles removed from the skin were comparable in size (area) and shape. Journal of Nature and Science (JNSCI), 2(2):e174, 2016

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